CATH Domain 5k1pA01

CATH Classification

Level	CATH Code	Description
	1	Mainly Alpha
	1.10	Orthogonal Bundle
	1.10.287	Helix Hairpins
	1.10.287.540	Helix hairpin bin

Domain Context

CATH Clusters

Superfamily	Helix hairpin bin
Functional Family

Enzyme Information

6.1.1.26

Pyrrolysine--tRNA(Pyl) ligase.

based on mapping to UniProt Q8PWY1

ATP + L-pyrrolysine + tRNA(Pyl) = AMP + diphosphate + L-pyrrolysyl- tRNA(Pyl).

-!- In organisms such as Methanosarcina barkeri that incorporate the modified amino acid pyrrolysine (Pyl) into certain methylamine methyltransferases, an unusual tRNA(Pyl), with a CUA anticodon, can be charged directly with pyrrolysine by this class II aminoacyl- -tRNA ligase. -!- Specific for pyrrolysine as substrate as it cannot be replaced by lysine or any of the other natural amino acids.

UniProtKB Entries (1)

Q8PWY1

PYLS_METMA

Methanosarcina mazei Go1

Pyrrolysine--tRNA ligase

PDB Structure

PDB	5K1P
External Links	PDBSum Proteopedia
Method	X-RAY DIFFRACTION
Organism
Primary Citation	Genetically encoded fluorophenylalanines enable insights into the recognition of lysine trimethylation by an epigenetic reader. Lee, Y.J., Schmidt, M.J., Tharp, J.M., Weber, A., Koenig, A.L., Zheng, H., Gao, J., Waters, M.L., Summerer, D., Liu, W.R. Chem.Commun.(Camb.)