CATH Domain 5i23A01

CATH Classification

Level	CATH Code	Description
	2	Mainly Beta
	2.60	Sandwich
	2.60.40	Immunoglobulin-like
	2.60.40.1760	glycosyl hydrolase (family 31)

Domain Context

CATH Clusters

Superfamily	glycosyl hydrolase (family 31)
Functional Family

Enzyme Information

2.4.1.161

Oligosaccharide 4-alpha-D-glucosyltransferase.

based on mapping to UniProt B3PEE6

Transfers the non-reducing terminal alpha-D-glucose residue from a (1->4)-alpha-D-glucan to the 4-position of a free glucose or of a glucosyl residue at the non-reducing terminus of a (1->4)-alpha-D-glucan, thus bringing about the rearrangement of oligosaccharides.

-!- The enzyme acts on amylose, amylopectin, glycogen and maltooligosaccharides. -!- No detectable free glucose is formed, indicating the enzyme does not act as a hydrolase. -!- The enzyme from the bacterium Cellvibrio japonicus has the highest activity with maltotriose as a donor, and also accepts maltose, while the enzyme from amoeba does not accept maltose. -!- Oligosaccharides with 1->6 linkages cannot function as donors, but can act as acceptors. -!- Unlike EC 2.4.1.25, this enzyme can transfer only a single glucosyl residue.

UniProtKB Entries (1)

B3PEE6

OL4AG_CELJU

Cellvibrio japonicus Ueda107

Oligosaccharide 4-alpha-D-glucosyltransferase

PDB Structure

PDB	5I23
External Links	PDBSum Proteopedia
Method	X-RAY DIFFRACTION
Organism
Primary Citation	Detection of Active Mammalian GH31 alpha-Glucosidases in Health and Disease Using In-Class, Broad-Spectrum Activity-Based Probes. Jiang, J., Kuo, C.L., Wu, L., Franke, C., Kallemeijn, W.W., Florea, B.I., van Meel, E., van der Marel, G.A., Codee, J.D., Boot, R.G., Davies, G.J., Overkleeft, H.S., Aerts, J.M. Acs Cent.Sci.