CATH Classification

Domain Context

CATH Clusters

Superfamily Rhomboid-like
Functional Family Rhomboid protease GlpG

Enzyme Information

3.4.21.105
Rhomboid protease.
based on mapping to UniProt P09391
Cleaves type-1 transmembrane domains using a catalytic dyad composed of serine and histidine that are contributed by different transmembrane domains.
-!- These endopeptidases are multi-spanning membrane proteins. -!- Their catalytic site is embedded within the membrane and they cleave type-1 transmembrane domains. -!- Important for embryo development in Drosophila melanogaster. -!- Rhomboid is a key regulator of EGF receptor signaling and is responsible for cleaving Spitz, the main ligand of the Drosophila EGF receptor pathway. -!- Parasite-encoded rhomboid enzymes are also important for invasion of host cells by Toxoplasma and the malaria parasite. -!- Belongs to peptidase family S54.

UniProtKB Entries (2)

P09391
GLPG_ECOLI
Escherichia coli K-12
Rhomboid protease GlpG
P42287
GRK_DROME
Drosophila melanogaster
Protein gurken

PDB Structure

PDB 5F5J
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Crystal Structures and Inhibition Kinetics Reveal a Two-Stage Catalytic Mechanism with Drug Design Implications for Rhomboid Proteolysis.
Cho, S., Dickey, S.W., Urban, S.
Mol.Cell