CATH Classification

Domain Context

CATH Clusters

Superfamily CCA tRNA nucleotidyltransferase, domain 2
Functional Family

Enzyme Information

2.7.7.72
CCA tRNA nucleotidyltransferase.
based on mapping to UniProt O28126
A tRNA precursor + 2 CTP + ATP = a tRNA with a 3' CCA end + 3 diphosphate.
-!- The acylation of all tRNAs with an amino acid occurs at the terminal ribose of a 3' CCA sequence. -!- The CCA sequence is added to the tRNA precursor by stepwise nucleotide addition performed by a single enzyme that is ubiquitous in all living organisms. -!- Although the enzyme has the option of releasing the product after each addition, it prefers to stay bound to the product and proceed with the next addition. -!- Formerly EC 2.7.7.21 and EC 2.7.7.25.

UniProtKB Entries (1)

O28126
CCA_ARCFU
Archaeoglobus fulgidus DSM 4304
CCA-adding enzyme

PDB Structure

PDB 4X4V
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
On-Enzyme Refolding Permits Small RNA and tRNA Surveillance by the CCA-Adding Enzyme.
Kuhn, C.D., Wilusz, J.E., Zheng, Y., Beal, P.A., Joshua-Tor, L.
Cell