CATH Classification

Domain Context

CATH Clusters

Superfamily Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
Functional Family Methylated-DNA--protein-cysteine methyltransferase

Enzyme Information

2.1.1.63
Methylated-DNA--[protein]-cysteine S-methyltransferase.
based on mapping to UniProt P9WJW5
(1) DNA (containing 6-O-methylguanine) + protein L-cysteine = DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine. (2) DNA (containing 4-O-methylthymine) + protein L-cysteine = DNA (without 4-O-methylthymine) + protein S-methyl-L-cysteine.
-!- This protein is involved in the repair of methylated DNA. -!- Unlike EC 3.2.2.20 and EC 3.2.2.21, which remove the methylated base leaving an apurinic/apyrimidinic site, this enzyme transfers the methyl group from the methylated DNA to an internal cysteine residue, leaving an intact nucleotide. -!- Since the methyl transfer is irreversible, the enzyme can only catalyze a single turnover.

UniProtKB Entries (1)

P9WJW5
OGT_MYCTU
Mycobacterium tuberculosis H37Rv
Methylated-DNA--protein-cysteine methyltransferase

PDB Structure

PDB 4WXC
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Crystal structure of Mycobacterium tuberculosis O6-methylguanine-DNA methyltransferase protein clusters assembled on to damaged DNA.
Miggiano, R., Perugino, G., Ciaramella, M., Serpe, M., Rejman, D., Pav, O., Pohl, R., Garavaglia, S., Lahiri, S., Rizzi, M., Rossi, F.
Biochem.J.