CATH Classification
Level | CATH Code | Description |
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3 | Alpha Beta |
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3.30 | 2-Layer Sandwich |
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3.30.1360 | Gyrase A; domain 2 |
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3.30.1360.30 | GAD-like domain |
Domain Context
CATH Clusters
Superfamily | GAD-like domain |
Functional Family |
Enzyme Information
6.1.1.23 |
Aspartate--tRNA(Asn) ligase.
based on mapping to UniProt Q51422
ATP + L-aspartate + tRNA(Asx) = AMP + diphosphate + L-aspartyl-tRNA(Asx).
-!- When this enzyme acts on tRNA(Asp), it catalyzes the same reaction as EC 6.1.1.12. -!- It has, however, diminished discrimination, so that it can also form aspartyl-tRNA(Asn). -!- This relaxation of specificity has been found to result from the absence of a loop in the tRNA that specifically recognizes the third position of the anticodon. -!- This accounts for the ability of this enzyme in, for example, Thermus thermophilus, to recognize both tRNA(Asp) (GUC anticodon) and tRNA(Asn) (GUU anticodon). -!- The aspartate-tRNA(Asn) is not used in protein synthesis until it is converted by EC 6.3.5.6 into asparaginyl-tRNA(Asn).
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UniProtKB Entries (1)
Q9HVT7 |
GATB_PSEAE
Pseudomonas aeruginosa PAO1
Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B
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PDB Structure
PDB | 4WJ3 |
External Links | |
Method | X-RAY DIFFRACTION |
Organism | |
Primary Citation |
Structure of the Pseudomonas aeruginosa transamidosome reveals unique aspects of bacterial tRNA-dependent asparagine biosynthesis
Proc.Natl.Acad.Sci.USA
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