CATH Classification

Domain Context

CATH Clusters

Superfamily Peptidase C65 Otubain, subdomain 1
Functional Family Ubiquitin thioesterase

Enzyme Information

3.4.19.12
Ubiquitinyl hydrolase 1.
based on mapping to UniProt Q96FW1
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
-!- Links to polypeptides smaller than 60 residues are hydrolyzed more readily than those to larger polypeptides. -!- Isoforms exist with quantitatively different specificities among the best known being UCH-L1 and UCH-L3, major proteins of the brain of mammals. -!- Inhibited by ubiquitin aldehyde (in which Gly76 is replaced by aminoacetaldehyde). -!- Belongs to peptidase family C12.
3.4.19.12
Ubiquitinyl hydrolase 1.
based on mapping to UniProt Q9XVR6
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
-!- Links to polypeptides smaller than 60 residues are hydrolyzed more readily than those to larger polypeptides. -!- Isoforms exist with quantitatively different specificities among the best known being UCH-L1 and UCH-L3, major proteins of the brain of mammals. -!- Inhibited by ubiquitin aldehyde (in which Gly76 is replaced by aminoacetaldehyde). -!- Belongs to peptidase family C12.

UniProtKB Entries (2)

Q9XVR6
OTUBL_CAEEL
Caenorhabditis elegans
Ubiquitin thioesterase otubain-like
P0CG48
UBC_HUMAN
Homo sapiens
Polyubiquitin-C

PDB Structure

PDB 4LDT
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
E2 ubiquitin-conjugating enzymes regulate the deubiquitinating activity of OTUB1.
Wiener, R., Dibello, A.T., Lombardi, P.M., Guzzo, C.M., Zhang, X., Matunis, M.J., Wolberger, C.
Nat.Struct.Mol.Biol.