CATH Domain 4kqxA02

CATH Classification

Level	CATH Code	Description
	6	Special
	6.10	Helix non-globular
	6.10.240	ProC C-terminal domain-like fold
	6.10.240.10

Domain Context

CATH Clusters

Superfamily	6.10.240.10
Functional Family

Enzyme Information

1.1.1.86

Ketol-acid reductoisomerase (NADP(+)).

based on mapping to UniProt D0WGK0

(1) (2R)-2,3-dihydroxy-3-methylbutanoate + NADP(+) = (2S)-2-hydroxy-2- methyl-3-oxobutanoate + NADPH. (2) (2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP(+) = (S)-2-hydroxy-2- ethyl-3-oxobutanoate + NADPH.

-!- The enzyme, found in many bacteria and archaea, is specific for NADPH (cf. EC 1.1.1.382 and EC 1.1.1.383). -!- Formerly EC 1.1.1.89.

UniProtKB Entries (1)

D0WGK0

ILVC_SLAES

Slackia exigua ATCC 700122

Ketol-acid reductoisomerase (NADP(+))

PDB Structure

PDB	4KQX
External Links	PDBSum Proteopedia
Method	X-RAY DIFFRACTION
Organism
Primary Citation	General approach to reversing ketol-acid reductoisomerase cofactor dependence from NADPH to NADH. Brinkmann-Chen, S., Flock, T., Cahn, J.K., Snow, C.D., Brustad, E.M., McIntosh, J.A., Meinhold, P., Zhang, L., Arnold, F.H. Proc.Natl.Acad.Sci.USA