CATH Domain 4iqiA02

CATH Classification

Level	CATH Code	Description
	3	Alpha Beta
	3.30	2-Layer Sandwich
	3.30.1130	GTP Cyclohydrolase I, domain 2
	3.30.1130.10	GTP cyclohydrolase I, C-terminal domain/NADPH-dependent 7-cyano-7-deazaguanine reductase, N-terminal domain

Domain Context

CATH Clusters

Superfamily	3.30.1130.10
Functional Family

Enzyme Information

1.7.1.13

PreQ(1) synthase.

based on mapping to UniProt Q9KTK0

7-aminomethyl-7-carbaguanine + 2 NADP(+) = 7-cyano-7-carbaguanine + 2 NADPH.

-!- The reaction occurs in the reverse direction. -!- This enzyme catalyzes one of the early steps in the synthesis of queosine (Q-tRNA), and is followed by the action of EC 2.4.2.29. -!- Queosine is found in the wobble position of tRNA(GUN) in eukaryotes and bacteria and is thought to be involved in translational modulation.

UniProtKB Entries (1)

Q9KTK0

QUEF_VIBCH

Vibrio cholerae O1 biovar El Tor str. N16961

NADPH-dependent 7-cyano-7-deazaguanine reductase

PDB Structure

PDB	4IQI
External Links	PDBSum Proteopedia
Method	X-RAY DIFFRACTION
Organism
Primary Citation	Crystal Structure of 7-cyano-7-deazaguanine Reductase, QueF from Vibrio cholerae O1 biovar El Tor complexed with cytosine Kim, Y., Zhou, M., Gu, M., Anderson, W.F., Joachimiak, A., Center for Structural Genomics of Infectious Diseases (CSGID) To be Published