CATH Domain 4ideA02

CATH Classification

Level	CATH Code	Description
	3	Alpha Beta
	3.40	3-Layer(aba) Sandwich
	3.40.50	Rossmann fold
	3.40.50.720	NAD(P)-binding Rossmann-like Domain

Domain Context

CATH Clusters

Superfamily	NAD(P)-binding Rossmann-like Domain
Functional Family

Enzyme Information

1.3.1.105

2-methylene-furan-3-one reductase.

based on mapping to UniProt O23939

4-hydroxy-2,5-dimethylfuran-3(2H)-one + NADP(+) = 4-hydroxy-5-methyl-2- methylenefuran-3(2H)-one + NADPH.

-!- The enzyme was dicovered in strawberry (Fragaria x ananassa), where it produces furaneol, one of the major aroma compounds in the fruits. -!- It has also been detected in tomato (Solanum lycopersicum) and pineapple (Ananas comosus). -!- The enzyme can also act on derivatives substituted at the methylene functional group. -!- The enzyme from the yeast Saccharomyces cerevisiae acts on (2E)-2- ethylidene-4-hydroxy-5-methylfuran-3(2H)-one and produces homofuraneol, an important aroma compound in soy sauce and miso. -!- NADPH is the preferred cofactor.

UniProtKB Entries (1)

O23939

ENOX_FRAVE

Fragaria vesca

2-methylene-furan-3-one reductase

PDB Structure

PDB	4IDE
External Links	PDBSum Proteopedia
Method	X-RAY DIFFRACTION
Organism
Primary Citation	Structural basis for the enzymatic formation of the key strawberry flavor compound 4-hydroxy-2,5-dimethyl-3(2H)-furanone Schiefner, A., Sinz, Q., Neumaier, I., Schwab, W., Skerra, A. J.Biol.Chem.