CATH Classification
Level | CATH Code | Description |
---|---|---|
3 | Alpha Beta | |
3.30 | 2-Layer Sandwich | |
3.30.560 | Glucose Oxidase; domain 3 | |
3.30.560.10 | Glucose Oxidase, domain 3 |
Domain Context
CATH Clusters
Superfamily | Glucose Oxidase, domain 3 |
Functional Family |
Enzyme Information
1.1.99.29 |
Pyranose dehydrogenase (acceptor).
based on mapping to UniProt Q3L245
(1) Pyranose + acceptor = pyranos-2-ulose + reduced acceptor. (2) Pyranose + acceptor = pyranos-3-ulose + reduced acceptor. (3) Pyranose + acceptor = pyranos-2,3-diulose + reduced acceptor. (4) A pyranoside + acceptor = a pyranosid-3-ulose + reduced acceptor. (5) A pyranoside + acceptor = a pyranosid-3,4-diulose + reduced acceptor.
-!- A number of aldoses and ketoses in pyranose form, as well as glycosides, gluco-oligosaccharides, sucrose and lactose can act as a donor. -!- 1,4-benzoquinone or ferricenium ion (ferrocene oxidized by removal of one electron) can serve as acceptor. -!- Unlike EC 1.1.3.10, this fungal enzyme does not interact with O(2) and exhibits extremely broad substrate tolerance with variable regioselectivity (C-3, C-2 or C-3 + C-2 or C-3 + C-4) for (di)oxidation of different sugars. -!- D-glucose is exclusively or preferentially oxidized at C-3 (depending on the enzyme source), but can also be oxidized at C-2 + C-3. -!- The enzyme also acts on 1->4-alpha- and 1->4-beta-gluco- oligosaccharides, non-reducing gluco-oligosaccharides and L-arabinose, which are not substrates of EC 1.1.3.10. -!- Sugars are oxidized in their pyranose but not in their furanose form.
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UniProtKB Entries (1)
Q3L245 |
PDH1_LEUMG
Leucoagaricus meleagris
Pyranose dehydrogenase 1
|
PDB Structure
PDB | 4H7U |
External Links | |
Method | X-RAY DIFFRACTION |
Organism | |
Primary Citation |
The 1.6 A crystal structure of pyranose dehydrogenase from Agaricus meleagris rationalizes substrate specificity and reveals a flavin intermediate.
Plos One
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