CATH Domain 4h6oA00

CATH Classification

Level	CATH Code	Description
	1	Mainly Alpha
	1.10	Orthogonal Bundle
	1.10.630	Cytochrome p450
	1.10.630.10	Cytochrome P450

Domain Context

CATH Clusters

Superfamily	Cytochrome P450
Functional Family

Enzyme Information

1.14.14.154

Sterol 14-alpha-demethylase.

based on mapping to UniProt Q7Z1V1

A 14-alpha-methylsteroid + 3 [reduced NADPH--hemoprotein reductase] + 3 O(2) = a Delta(14)-steroid + formate + 3 [oxidized NADPH--hemoprotein reductase] + 4 H(2)O.

-!- This cytochrome P450 (heme-thiolate) enzyme acts on a range of steroids with a 14-alpha-methyl group, such as obtusifoliol and lanosterol. -!- The enzyme catalyzes a hydroxylation and a reduction of the 14-alpha- methyl group, followed by a second hydroxylation, resulting in the elimination of formate and formation of a 14(15) double bond. -!- Formerly EC 1.14.13.70.

UniProtKB Entries (1)

Q7Z1V1

CP51_TRYCC

Trypanosoma cruzi strain CL Brener

Sterol 14-alpha demethylase

PDB Structure

PDB	4H6O
External Links	PDBSum Proteopedia
Method	X-RAY DIFFRACTION
Organism
Primary Citation	Antitrypanosomal Lead Discovery: Identification of a Ligand-Efficient Inhibitor of Trypanosoma cruzi CYP51 and Parasite Growth. Andriani, G., Amata, E., Beatty, J., Clements, Z., Coffey, B.J., Courtemanche, G., Devine, W., Erath, J., Juda, C.E., Wawrzak, Z., Wood, J.T., Lepesheva, G.I., Rodriguez, A., Pollastri, M.P. J.Med.Chem.