CATH Domain 4gynA00

CATH Classification

Level	CATH Code	Description
	3	Alpha Beta
	3.60	4-Layer Sandwich
	3.60.110	Nitrilase/N-carbamoyl-D-aminoacid amidohydrolase
	3.60.110.10	Carbon-nitrogen hydrolase

Domain Context

CATH Clusters

Superfamily	Carbon-nitrogen hydrolase
Functional Family

Enzyme Information

3.5.1.4

Amidase.

based on mapping to UniProt Q9L543

A monocarboxylic acid amide + H(2)O = a monocarboxylate + NH(3).

UniProtKB Entries (1)

AMIE_BACSP

Bacillus sp. (in: Bacteria)

Aliphatic amidase

PDB Structure

PDB	4GYN
External Links	PDBSum Proteopedia
Method	X-RAY DIFFRACTION
Organism
Primary Citation	The mechanism of the amidases: mutating the glutamate adjacent to the catalytic triad inactivates the enzyme due to substrate mispositioning. Weber, B.W., Kimani, S.W., Varsani, A., Cowan, D.A., Hunter, R., Venter, G.A., Gumbart, J.C., Sewell, B.T. J.Biol.Chem.