CATH Classification

Domain Context

CATH Clusters

Superfamily 3.30.1360.170
Functional Family

Enzyme Information

2.1.1.148
Thymidylate synthase (FAD).
based on mapping to UniProt Q9WYT0
5,10-methylenetetrahydrofolate + dUMP + NADPH = dTMP + tetrahydrofolate + NADP(+).
-!- Contains FAD. -!- All thymidylate synthases catalyze a reductive methylation involving the transfer of the methylene group of 5,10-methylenetetrahydrofolate to the C5-position of dUMP and a two electron reduction of the methylene group to a methyl group. -!- Unlike the classical thymidylate synthase, ThyA (EC 2.1.1.45), which uses folate as both a 1-carbon donor and a source of reducing equivalents, this enzyme uses a flavin coenzyme as a source of reducing equivalents, which are derived from NADPH.

UniProtKB Entries (1)

Q9WYT0
THYX_THEMA
Thermotoga maritima MSB8
Flavin-dependent thymidylate synthase

PDB Structure

PDB 4GTL
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Folate binding site of flavin-dependent thymidylate synthase.
Koehn, E.M., Perissinotti, L.L., Moghram, S., Prabhakar, A., Lesley, S.A., Mathews, I.I., Kohen, A.
Proc.Natl.Acad.Sci.USA
CATH-Gene3D is a Global Biodata Core Resource Learn more...