CATH Domain 4g8vA00

CATH Classification

Level	CATH Code	Description
	3	Alpha Beta
	3.10	Roll
	3.10.130	P-30 Protein
	3.10.130.10	Ribonuclease A-like domain

Domain Context

CATH Clusters

Superfamily	Ribonuclease A-like domain
Functional Family	Ribonuclease pancreatic

Enzyme Information

4.6.1.18

Pancreatic ribonuclease.

based on mapping to UniProt P61823

(1) An (RNA) containing cytidine + H(2)O = an (RNA)-3'-cytidine- 3'-phosphate + a 5'-hydroxy-ribonucleotide-3'-(RNA). (2) An (RNA) containing uridine + H(2)O = an (RNA)-3'-uridine- 3'-phosphate + a 5'-hydroxy-ribonucleotide-3'-(RNA).

-!- Specifically cleaves at the 3'-side of pyrimidine (uracil or cytosine) phosphate bonds in RNA. -!- The reaction takes place in two steps, with the 2',3'-cyclic phosphodiester intermediates released from the enzyme at the completion of the first step. -!- Hydrolysis of these cyclic compounds occurs at a much slower rate through a reversal of the first step, in which the -OH group of water substitutes for the 2'-OH group of the ribose used in the first step, and does not take place until essentially all the susceptible 3',5'-phosphodiester bonds have been cyclised. -!- The enzyme can act as an endo- or exo ribonuclease. -!- Formerly EC 2.7.7.16, EC 3.1.4.22 and EC 3.1.27.5.

UniProtKB Entries (1)

P61823

RNAS1_BOVIN

Bos taurus

Ribonuclease pancreatic

PDB Structure

PDB	4G8V
External Links	PDBSum Proteopedia
Method	X-RAY DIFFRACTION
Organism
Primary Citation	Triazole pyrimidine nucleosides as inhibitors of Ribonuclease A. Synthesis, biochemical, and structural evaluation. Parmenopoulou, V., Chatzileontiadou, D.S., Manta, S., Bougiatioti, S., Maragozidis, P., Gkaragkouni, D.N., Kaffesaki, E., Kantsadi, A.L., Skamnaki, V.T., Zographos, S.E., Zounpoulakis, P., Balatsos, N.A., Komiotis, D., Leonidas, D.D. Bioorg.Med.Chem.