CATH Domain 4f8bA00

CATH Classification

Level	CATH Code	Description
	3	Alpha Beta
	3.30	2-Layer Sandwich
	3.30.1130	GTP Cyclohydrolase I, domain 2
	3.30.1130.10	GTP cyclohydrolase I, C-terminal domain/NADPH-dependent 7-cyano-7-deazaguanine reductase, N-terminal domain

Domain Context

CATH Clusters

Superfamily	3.30.1130.10
Functional Family

Enzyme Information

1.7.1.13

PreQ(1) synthase.

based on mapping to UniProt O31678

7-aminomethyl-7-carbaguanine + 2 NADP(+) = 7-cyano-7-carbaguanine + 2 NADPH.

-!- The reaction occurs in the reverse direction. -!- This enzyme catalyzes one of the early steps in the synthesis of queosine (Q-tRNA), and is followed by the action of EC 2.4.2.29. -!- Queosine is found in the wobble position of tRNA(GUN) in eukaryotes and bacteria and is thought to be involved in translational modulation.

UniProtKB Entries (1)

O31678

QUEF_BACSU

Bacillus subtilis subsp. subtilis str. 168

NADPH-dependent 7-cyano-7-deazaguanine reductase

PDB Structure

PDB	4F8B
External Links	PDBSum Proteopedia
Method	X-RAY DIFFRACTION
Organism
Primary Citation	Structural basis of biological nitrile reduction. Chikwana, V.M., Stec, B., Lee, B.W., de Crecy-Lagard, V., Iwata-Reuyl, D., Swairjo, M.A. J.Biol.Chem.