CATH Domain 4d13A00

CATH Classification

Level	CATH Code	Description
	3	Alpha Beta
	3.10	Roll
	3.10.270	Urate Oxidase
	3.10.270.10	Urate Oxidase;

Domain Context

CATH Clusters

Superfamily	Urate Oxidase;
Functional Family	Uricase

Enzyme Information

1.7.3.3

Factor independent urate hydroxylase.

based on mapping to UniProt Q00511

Urate + O(2) + H(2)O = 5-hydroxyisourate + H(2)O(2).

-!- Was previously thought to be a copper protein, but it is now known that the enzymes from soybean (Glycine max), the mold Aspergillus flavus and Bacillus subtilis contains no copper nor any other transition-metal ion. -!- The 5-hydroxyisourate formed decomposes spontaneously to form allantoin and CO(2), although there is an enzyme-catalyzed pathway in which EC 3.5.2.17 catalyzes the first step. -!- The enzyme is different from EC 1.14.13.113.

UniProtKB Entries (1)

Q00511

URIC_ASPFL

Aspergillus flavus

Uricase

PDB Structure

PDB	4D13
External Links	PDBSum Proteopedia
Method	X-RAY DIFFRACTION
Organism
Primary Citation	Direct evidence for a peroxide intermediate and a reactive enzyme-substrate-dioxygen configuration in a cofactor-free oxidase. Bui, S., von Stetten, D., Jambrina, P.G., Prange, T., Colloc'h, N., de Sanctis, D., Royant, A., Rosta, E., Steiner, R.A. Angew. Chem. Int. Ed. Engl.