CATH Classification
| Level | CATH Code | Description |
|---|---|---|
|
3 | Alpha Beta |
|
3.40 | 3-Layer(aba) Sandwich |
|
3.40.1280 | Alpha/beta knot |
|
3.40.1280.10 | SPOUT methyltransferase, trefoil knot domain |
Domain Context
CATH Clusters
| Superfamily | 3.40.1280.10 |
| Functional Family | Uncharacterized tRNA/rRNA methyltransferase HI_0380 |
Enzyme Information
| 2.1.1.200 |
tRNA (cytidine(32)/uridine(32)-2'-O)-methyltransferase.
based on mapping to UniProt P0AE01
(1) S-adenosyl-L-methionine + cytidine(32) in tRNA = S-adenosyl-L- homocysteine + 2'-O-methylcytidine(32) in tRNA. (2) S-adenosyl-L-methionine + uridine(32) in tRNA = S-adenosyl-L- homocysteine + 2'-O-methyluridine(32) in tRNA.
-!- In Escherichia coli TrmJ is the only methyltransferase responsible for the formation of 2'-O-methylcytidine(32) in tRNA. -!- No methylation of cytosine(34) in tRNA(Leu)(CAA). -!- In vitro the enzyme 2'-O-methylates cytidine(34) of tRNA(Ser1) and uridine(32) of tRNA(Gln2).
|
UniProtKB Entries (1)
| P0AE01 |
TRMJ_ECOLI
Escherichia coli K-12
TRNA (cytidine/uridine-2'-O-)-methyltransferase TrmJ
|
PDB Structure
| PDB | 4CND |
| External Links | |
| Method | X-RAY DIFFRACTION |
| Organism | |
| Primary Citation |
Characterization of Two Homologous 2'-O-Methyltransferases Showing Different Specificities for Their tRNA Substrates.
RNA
|
