CATH Domain 4c2zA00

CATH Classification

Level	CATH Code	Description
	3	Alpha Beta
	3.40	3-Layer(aba) Sandwich
	3.40.630	Aminopeptidase
	3.40.630.170

Domain Context

CATH Clusters

Superfamily	3.40.630.170
Functional Family	Glycylpeptide N-tetradecanoyltransferase

Enzyme Information

2.3.1.97

Glycylpeptide N-tetradecanoyltransferase.

based on mapping to UniProt P30419

Tetradecanoyl-CoA + an N-terminal-glycyl-[protein] = CoA + an N-terminal- N-tetradecanoylglycyl-[protein].

-!- The enzyme catalyzes the transfer of myristic acid from myristoyl-CoA to the amino group of the N-terminal glycine residue in a variety of eukaryotic proteins. -!- It uses an ordered Bi Bi reaction in which myristoyl-CoA binds to the enzyme prior to the binding of the peptide substrate, and CoA release precedes the release of the myristoylated peptide. -!- The enzyme from yeast is profoundly affected by amino acids further from the N-terminus, and is particularly stimulated by a serine residue at position 5.

UniProtKB Entries (1)

P30419

NMT1_HUMAN

Homo sapiens

Glycylpeptide N-tetradecanoyltransferase 1

PDB Structure

PDB	4C2Z
External Links	PDBSum Proteopedia
Method	X-RAY DIFFRACTION
Organism
Primary Citation	Chemical Proteomics Defines the Mammalian N- Myristoylated Proteome in Live Cells Global Profiling of Co- and Post-Translationally N-Myristoylated Proteomes in Human Cells. Thinon, E., Serwa, R.A., Broncel, M., Brannigan, J.A., Brassat, U., Wright, M.H., Heal, W.P., Wilkinson, A.J., Mann, D.J., Tate, E.W. Nat.Commun.