CATH Domain 4ay5A03

CATH Classification

Level	CATH Code	Description
	3	Alpha Beta
	3.40	3-Layer(aba) Sandwich
	3.40.50	Rossmann fold
	3.40.50.11380

Domain Context

CATH Clusters

Superfamily	3.40.50.11380
Functional Family	UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit

Enzyme Information

2.4.1.255

Protein O-GlcNAc transferase.

based on mapping to UniProt O15294

(1) UDP-N-acetyl-alpha-D-glucosamine + [protein]-L-serine = UDP + [protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-serine. (2) UDP-N-acetyl-alpha-D-glucosamine + [protein]-L-threonine = UDP + [protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-threonine.

-!- Within higher eukaryotes post-translational modification of protein serines/threonines with N-acetylglucosamine (O-GlcNAc) is dynamic, inducible and abundant, regulating many cellular processes by interfering with protein phosphorylation. -!- EC 2.4.1.255 transfers GlcNAc onto substrate proteins and EC 3.2.1.169 cleaves GlcNAc from the modified proteins.

UniProtKB Entries (1)

Q15750

TAB1_HUMAN

Homo sapiens

TGF-beta-activated kinase 1 and MAP3K7-binding protein 1

PDB Structure

PDB	4AY5
External Links	PDBSum Proteopedia
Method	X-RAY DIFFRACTION
Organism
Primary Citation	O-Glcnac Transferase Invokes Nucleotide Sugar Pyrophosphate Participation in Catalysis Schimpl, M., Zheng, X., Borodkin, V.S., Blair, D.E., Ferenbach, A.T., Schuettelkopf, A.W., Navratilova, I., Aristotelous, T., Albarbarawi, O., Robinson, D.A., Macnaughtan, M.A., Van Aalten, D.M.F. Nat.Chem.Biol.