CATH Domain 4ae1A02

CATH Classification

Level	CATH Code	Description
	1	Mainly Alpha
	1.10	Orthogonal Bundle
	1.10.490	Globin-like
	1.10.490.40	Diphtheria toxin, translocation domain

Domain Context

CATH Clusters

Superfamily	Diphtheria toxin, translocation domain
Functional Family	Diphtheria toxin

Enzyme Information

2.4.2.36

NAD(+)--diphthamide ADP-ribosyltransferase.

based on mapping to UniProt P00588

NAD(+) + diphthamide-[translation elongation factor 2] = nicotinamide + N-(ADP-D-ribosyl)diphthamide-[translation elongation factor 2].

-!- Dihtheria toxin and some other bacterial toxins catalyze this reaction, which inactivates translation elongation factor 2 (EF2). -!- The acceptor is diphthamide, a unique modification of a histidine residue in the elongation factor found in archaebacteria and all eukaryotes, but not in eubacteria. -!- Cf. EC 2.4.2.31. -!- The relevant histidine of EF2 is His(715) in mammals, His(699) in yeast and His(600) in Pyrococcus horikoshii.

UniProtKB Entries (1)

P00588

DTX_CORBE

Corynephage beta

Diphtheria toxin

PDB Structure

PDB	4AE1
External Links	PDBSum Proteopedia
Method	X-RAY DIFFRACTION
Organism
Primary Citation	Structural Basis for Lack of Toxicity of the Diphtheria Toxin Mutant Crm197. Malito, E., Bursulaya, B., Chen, C., Surdo, P.L., Picchianti, M., Balducci, E., Biancucci, M., Brock, A., Berti, F., Bottomley, M.J., Nissum, M., Costantino, P., Rappuoli, R., Spraggon, G. Proc.Natl.Acad.Sci.USA