CATH Classification
Domain Context
CATH Clusters
| Superfamily | 3.90.1200.10 |
| Functional Family |
Enzyme Information
| 2.7.1.163 |
Hygromycin B 4-O-kinase.
based on mapping to UniProt P00557
ATP + hygromycin B = ADP + 4-O-phosphohygromycin B.
-!- Phosphorylates the antibiotic hygromycin B. -!- Whereas the enzyme from Streptomyces hygroscopicus (EC 2.7.1.119) catalyzes the formation of 7''-O-phosphohygromycin B, this enzyme, found in Escherichia coli carrying a plasmid conferring resistance to hygromycin-B, forms 4-O-phosphohygromycin B. -!- Formerly EC 2.7.1.n6.
|
UniProtKB Entries (1)
| P00557 |
KHYB_ECOLX
Escherichia coli
Hygromycin-B 4-O-kinase
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PDB Structure
| PDB | 3W0R |
| External Links | |
| Method | X-RAY DIFFRACTION |
| Organism | |
| Primary Citation |
Crystal structures of the ternary complex of APH(4)-Ia/Hph with hygromycin B and an ATP analog using a thermostable mutant.
J.Struct.Biol.
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