CATH Classification

Domain Context

CATH Clusters

Superfamily Ribonucleotide Reductase, subunit A
Functional Family Ribonucleoside-diphosphate reductase subunit M2 B

Enzyme Information

1.17.4.1
Ribonucleoside-diphosphate reductase.
based on mapping to UniProt P31350
2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H(2)O = ribonucleoside diphosphate + thioredoxin.
-!- This enzyme is responsible for the de novo conversion of ribonucleoside diphosphates into deoxyribonucleoside diphosphates, which are essential for DNA synthesis and repair. -!- There are three types of this enzyme differing in their cofactors. -!- Class Ia enzymes contain a diiron(III)-tyrosyl radical, class Ib enzymes contain a dimanganese-tyrosyl radical, and class II enzymes contain adenosylcobalamin. -!- In all cases the cofactors are involved in generation of a transient thiyl (sulfanyl) radical on a cysteine residue, which attacks the substrate, forming a ribonucleotide 3'-radical, followed by water loss to form a ketyl (alpha-oxoalkyl) radical. -!- The ketyl radical is reduced to 3'-keto-deoxynucleotide concomitant with formation of a disulfide anion radical between two cysteine residues. -!- A proton-coupled electron-transfer from the disulfide radical to the substrate generates a 3'-deoxynucleotide radical, and the the final product is formed when the hydrogen atom that was initially removed from the 3'-position of the nucleotide by the thiyl radical is returned to the same position. -!- The disulfide bridge is reduced by the action of thioredoxin. -!- Cf. EC 1.1.98.6 and EC 1.17.4.2.

UniProtKB Entries (1)

P31350
RIR2_HUMAN
Homo sapiens
Ribonucleoside-diphosphate reductase subunit M2

PDB Structure

PDB 3VPO
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Essential role of E106 in the proton-coupled electron transfer in human
Chen, X., Xu, Z., Liu, H., Zhang, L., Chen, B., Zhu, L., Yang, C., Zhu, W., Shao, J.
to be published