CATH Classification

Domain Context

CATH Clusters

Superfamily Peptidase C65 Otubain, subdomain 2
Functional Family Ubiquitin thioesterase OTUB1

Enzyme Information

3.4.19.12
Ubiquitinyl hydrolase 1.
based on mapping to UniProt Q96FW1
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
-!- Links to polypeptides smaller than 60 residues are hydrolyzed more readily than those to larger polypeptides. -!- Isoforms exist with quantitatively different specificities among the best known being UCH-L1 and UCH-L3, major proteins of the brain of mammals. -!- Inhibited by ubiquitin aldehyde (in which Gly76 is replaced by aminoacetaldehyde). -!- Belongs to peptidase family C12.

UniProtKB Entries (1)

P61088
UBE2N_HUMAN
Homo sapiens
Ubiquitin-conjugating enzyme E2 N

PDB Structure

PDB 3VON
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Molecular basis of K63-linked polyubiquitination inhibition by the interaction between human deubiquitinating enzyme OTUB1 and ubiquitin-conjugating enzyme UBC13
Sato, Y., Yamagata, A., Goto-Ito, S., Kubota, K., Miyamoto, K., Nakada, S., Fukai, S.
To be Published