CATH Domain 3t4vA00

CATH Classification

Level	CATH Code	Description
	2	Mainly Beta
	2.60	Sandwich
	2.60.120	Jelly Rolls
	2.60.120.590	Alpha-ketoglutarate-dependent dioxygenase AlkB-like

Domain Context

CATH Clusters

Superfamily	Alpha-ketoglutarate-dependent dioxygenase AlkB-like
Functional Family	Alpha-ketoglutarate-dependent dioxygenase AlkB

Enzyme Information

1.14.11.33

DNA oxidative demethylase.

based on mapping to UniProt P05050

DNA-base-CH(3) + 2-oxoglutarate + O(2) = DNA-base + formaldehyde + succinate + CO(2).

-!- Activity is slightly stimulated by ascorbate. -!- Catalyzes oxidative demethylation of the DNA base lesions N(1)- methyladenine, N(3)-methylcytosine, N(1)-methylguanine, and N(3)- methylthymine. -!- It works better on single-stranded DNA (ssDNA) and is capable of repairing damaged bases in RNA.

UniProtKB Entries (1)

P05050

ALKB_ECOLI

Escherichia coli K-12

Alpha-ketoglutarate-dependent dioxygenase AlkB

PDB Structure

PDB	3T4V
External Links	PDBSum Proteopedia
Method	X-RAY DIFFRACTION
Organism
Primary Citation	Dynamic combinatorial mass spectrometry leads to inhibitors of a 2-oxoglutarate-dependent nucleic Acid demethylase. Woon, E.C., Demetriades, M., Bagg, E.A., Aik, W., Krylova, S.M., Ma, J.H., Chan, M., Walport, L.J., Wegman, D.W., Dack, K.N., McDonough, M.A., Krylov, S.N., Schofield, C.J. J.Med.Chem.