CATH Domain 3rz3A00

CATH Classification

Level	CATH Code	Description
	3	Alpha Beta
	3.10	Roll
	3.10.110	Ubiquitin Conjugating Enzyme
	3.10.110.10	Ubiquitin Conjugating Enzyme

Domain Context

CATH Clusters

Superfamily	Ubiquitin Conjugating Enzyme
Functional Family	Ubiquitin-conjugating enzyme E2 R2

Enzyme Information

2.3.2.23

E2 ubiquitin-conjugating enzyme.

based on mapping to UniProt P49427

S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L- cysteine.

-!- The E2 ubiquitin-conjugating enzyme acquires the activated ubquitin from the E1 ubiquitin-activating enzyme (EC 6.2.1.45) and binds it via a transthioesterification reaction to itself. -!- In the human enzyme the catalytic center is located at Cys-87 where ubiquitin is bound via its C-terminal glycine in a thioester linkage. -!- Formerly EC 6.3.2.19.

2.3.2.24

(E3-independent) E2 ubiquitin-conjugating enzyme.

based on mapping to UniProt P49427

S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E1 ubiquitin-activating enzyme]-L-cysteine + N(6)- monoubiquitinyl-[acceptor protein]-L-lysine.

-!- The enzyme transfers a single ubiquitin directly from an ubiquitinated E1 ubiquitin-activating enzyme to itself, and on to a lysine residue of the acceptor protein without involvement of E3 ubiquitin transferases (cf. EC 2.3.2.26, EC 2.3.2.27). -!- It forms a labile ubiquitin adduct in the presence of E1, ubiquitin, and Mg(2+)-ATP and catalyzes the conjugation of ubiquitin to protein substrates, independently of E3. -!- This transfer has only been observed with small proteins. -!- In vitro a transfer to small acceptors (e.g. L-lysine, N-acetyl-L- lysine methyl ester) has been observed.

UniProtKB Entries (1)

P49427

UB2R1_HUMAN

Homo sapiens

Ubiquitin-conjugating enzyme E2 R1

PDB Structure

PDB	3RZ3
External Links	PDBSum Proteopedia
Method	X-RAY DIFFRACTION
Organism
Primary Citation	An allosteric inhibitor of the human cdc34 ubiquitin conjugating enzyme Ceccarelli, D.F., Tang, X., Pelletier, B., Orlicky, S., Xie, W., Plantevin, V., Neculai, D., Chou, Y.C., Ogunjimi, A., Al-Hakim, A., Varelas, X., Koszela, J., Wasney, G.A., Vedadi, M., Dhe-Paganon, S., Cox, S., Xu, S., Lopez-Girona, A., Mercurio, F., Wrana, J., Durocher, D., Meloche, S., Webb, D.R., Tyers, M., Sicheri, F. Cell(Cambridge,Mass.)