CATH Domain 3pneA03

CATH Classification

Level	CATH Code	Description
	3	Alpha Beta
	3.90	Alpha-Beta Complex
	3.90.1230	Bovine Endothelial Nitric Oxide Synthase Heme Domain; Chain: A,domain 3
	3.90.1230.10	Nitric Oxide Synthase; Chain A, domain 3

Domain Context

CATH Clusters

Superfamily	Nitric Oxide Synthase; Chain A, domain 3
Functional Family	Nitric oxide synthase, brain

Enzyme Information

1.14.13.39

Nitric-oxide synthase (NADPH).

based on mapping to UniProt P29476

2 L-arginine + 3 NADPH + 4 O(2) = 2 L-citrulline + 2 nitric oxide + 3 NADP(+) + 4 H(2)O.

-!- The enzyme consists of linked oxygenase and reductase domains. -!- The eukaryotic enzyme binds FAD, FMN, heme (iron protoporphyrin IX) and tetrahydrobiopterin, and its two domains are linked via a regulatory calmodulin-binding domain. -!- Upon calcium-induced calmodulin binding, the reductase and oxygenase domains form a complex, allowing electrons to flow from NADPH via FAD and FMN to the active center. -!- The reductase domain of the enzyme from the bacterium Sorangium cellulosum utilizes a [2Fe-2S] cluster to transfer the electrons from NADPH to the active center. -!- Cf. EC 1.14.14.47.

UniProtKB Entries (1)

P29476

NOS1_RAT

Rattus norvegicus

Nitric oxide synthase, brain

PDB Structure

PDB	3PNE
External Links	PDBSum Proteopedia
Method	X-RAY DIFFRACTION
Organism
Primary Citation	Improved synthesis of chiral pyrrolidine inhibitors and their binding properties to neuronal nitric oxide synthase. Xue, F., Kraus, J.M., Labby, K.J., Ji, H., Mataka, J., Xia, G., Li, H., Delker, S.L., Roman, L.J., Martasek, P., Poulos, T.L., Silverman, R.B. J.Med.Chem.