CATH Domain 3oinA01

CATH Classification

Level	CATH Code	Description
	3	Alpha Beta
	3.40	3-Layer(aba) Sandwich
	3.40.1280	Alpha/beta knot
	3.40.1280.10	SPOUT methyltransferase, trefoil knot domain

Domain Context

CATH Clusters

Superfamily	3.40.1280.10
Functional Family	Ribosomal RNA small subunit methyltransferase

Enzyme Information

2.1.1.260

rRNA small subunit pseudouridine methyltransferase Nep1.

based on mapping to UniProt Q06287

S-adenosyl-L-methionine + pseudouridine(1191) in yeast 18S rRNA = S-adenosyl-L-homocysteine + N(1)-methylpseudouridine(1191) in yeast 18S rRNA.

-!- This enzyme, which occurs in both prokaryotes and eukaryotes, recognizes specific pseudouridine residues (Psi) in small subunits of ribosomal RNA based on the local RNA structure. -!- It recognizes Psi(914) in 16S rRNA from the archaeon Methanocaldococcus jannaschii, Psi(1191) in yeast 18S rRNA, and Psi(1248) in human 18S rRNA.

UniProtKB Entries (1)

Q06287

NEP1_YEAST

Saccharomyces cerevisiae S288C

Ribosomal RNA small subunit methyltransferase NEP1

PDB Structure

PDB	3OIN
External Links	PDBSum Proteopedia
Method	X-RAY DIFFRACTION
Organism
Primary Citation	Structural insight into the functional mechanism of Nep1/Emg1 N1-specific pseudouridine methyltransferase in ribosome biogenesis. Thomas, S.R., Keller, C.A., Szyk, A., Cannon, J.R., Laronde-Leblanc, N.A. Nucleic Acids Res.