CATH Classification

Domain Context

CATH Clusters

Superfamily 3.40.1280.10
Functional Family Ribosomal RNA small subunit methyltransferase

Enzyme Information

2.1.1.260
rRNA small subunit pseudouridine methyltransferase Nep1.
based on mapping to UniProt Q06287
S-adenosyl-L-methionine + pseudouridine(1191) in yeast 18S rRNA = S-adenosyl-L-homocysteine + N(1)-methylpseudouridine(1191) in yeast 18S rRNA.
-!- This enzyme, which occurs in both prokaryotes and eukaryotes, recognizes specific pseudouridine residues (Psi) in small subunits of ribosomal RNA based on the local RNA structure. -!- It recognizes Psi(914) in 16S rRNA from the archaeon Methanocaldococcus jannaschii, Psi(1191) in yeast 18S rRNA, and Psi(1248) in human 18S rRNA.

UniProtKB Entries (1)

Q06287
NEP1_YEAST
Saccharomyces cerevisiae S288C
Ribosomal RNA small subunit methyltransferase NEP1

PDB Structure

PDB 3OIJ
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Structural insight into the functional mechanism of Nep1/Emg1 N1-specific pseudouridine methyltransferase in ribosome biogenesis.
Thomas, S.R., Keller, C.A., Szyk, A., Cannon, J.R., Laronde-Leblanc, N.A.
Nucleic Acids Res.