CATH Classification

Domain Context

CATH Clusters

Superfamily Aldolase class I
Functional Family Thiamine-phosphate synthase

Enzyme Information

2.5.1.3
Thiamine phosphate synthase.
based on mapping to UniProt P9WG75
(1) 4-amino-2-methyl-5-diphosphomethylpyrimidine + 2-((2R,5Z)-2-carboxy- 4-methylthiazol-5(2H)-ylidene)ethyl phosphate = diphosphate + thiamine phosphate + CO(2). (2) 4-amino-2-methyl-5-diphosphomethylpyrimidine + 2-(2-carboxy-4- methylthiazol-5-yl)ethyl phosphate = diphosphate + thiamine phosphate + CO(2). (3) 4-amino-2-methyl-5-diphosphomethylpyrimidine + 4-methyl-5- (2-phosphono-oxyethyl)thiazole = diphosphate + thiamine phosphate.
-!- The enzyme catalyzes the penultimate reaction in thiamine de novo biosynthesis, condensing the pyrimidine and thiazole components. -!- The enzyme is thought to accept the product of EC 2.8.1.10 as its substrate. -!- However, it has been shown that in some bacteria, such as Bacillus subtilis, an additional enzyme, EC 5.3.99.10 converts that compound into its tautomer 2-(2-carboxy-4-methylthiazol-5-yl)ethyl phosphate, and that it is the latter that serves as the substrate for the synthase. -!- In addition to this activity, the enzyme participates in a salvage pathway, acting on 4-methyl-5-(2-phosphono-oxyethyl)thiazole, which is produced from thiamine degradation products. -!- In yeast this activity is found in a bifunctional enzyme and in the plant Arabidopsis thaliana the activity is part of a trifunctional enzyme.

UniProtKB Entries (1)

P9WG75
THIE_MYCTU
Mycobacterium tuberculosis H37Rv
Thiamine-phosphate synthase

PDB Structure

PDB 3O63
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Crystal Structure and Identification of Potential Inhibitor Compounds for Mycobacterium tuberculosis Thiamin Phosphate Synthase
McCulloch, K.M., Ramamoorthy, D., Ishida, K., Guida, W.C., Begley, T.P., Ealick, S.E.
to be published
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