CATH Domain 3kv4A01

CATH Classification

Level	CATH Code	Description
	2	Mainly Beta
	2.60	Sandwich
	2.60.120	Jelly Rolls
	2.60.120.650	Cupin

Domain Context

CATH Clusters

Superfamily	Cupin
Functional Family	histone lysine demethylase PHF8 isoform X1

Enzyme Information

1.14.11.27

[Histone H3]-dimetyl-L-lysine-36 demethylase.

based on mapping to UniProt Q9UPP1

[Protein]-N(6),N(6)-dimethyl-L-lysine + 2 2-oxoglutarate + 2 O(2) = [protein]-L-lysine + 2 succinate + 2 formaldehyde + 2 CO(2).

-!- Of the seven potential methylation sites in histones H3 (K4, K9, K27, K36, K79) and H4 (K20, R3) from HeLa cells, the enzyme is specific for Lys-36. -!- Lysine residues exist in three methylation states (mono-, di- and trimethylated). -!- The enzyme preferentially demethylates the dimethyl form of Lys-36 (K36me2), which is its natural substrate, to form the monomethyl and unmethylated forms of Lys-36. -!- It can also demethylate the monomethyl- but not the trimethyl form of Lys-36.

UniProtKB Entries (1)

Q9UPP1

PHF8_HUMAN

Homo sapiens

Histone lysine demethylase PHF8

PDB Structure

PDB	3KV4
External Links	PDBSum Proteopedia
Method	X-RAY DIFFRACTION
Organism
Primary Citation	Enzymatic and structural insights for substrate specificity of a family of jumonji histone lysine demethylases. Horton, J.R., Upadhyay, A.K., Qi, H.H., Zhang, X., Shi, Y., Cheng, X. Nat.Struct.Mol.Biol.