CATH Domain 3h0jA02

CATH Classification

Level	CATH Code	Description
	2	Mainly Beta
	2.40	Beta Barrel
	2.40.460	ClpP/crotonase fold
	2.40.460.10	Biotin dependent carboxylase carboxyltransferase

Domain Context

CATH Clusters

Superfamily	Biotin dependent carboxylase carboxyltransferase
Functional Family	Acetyl-CoA carboxylase 1

Enzyme Information

6.3.4.14

Biotin carboxylase.

based on mapping to UniProt Q00955

ATP + [biotin carboxyl-carrier protein]-biotin-N(6)-L-lysine + hydrogencarbonate- = ADP + phosphate + [biotin carboxyl-carrier protein]- carboxybiotin-N(6)-L-lysine.

-!- This enzyme, part of an acetyl-CoA carboxylase complex, acts on a biotin carboxyl-carrier protein (BCCP) that has been biotinylated by EC 6.3.4.15. -!- In some organisms the enzyme is part of a multi-domain polypeptide that also includes the carrier protein (e.g. mycobacteria). -!- Yet in other organisms (e.g. mammals) this activity is included in a single polypeptide that also catalyzes the transfer of the carboxyl group from biotin to acetyl-CoA (see EC 6.4.1.2).

6.4.1.2

Acetyl-CoA carboxylase.

based on mapping to UniProt Q00955

ATP + acetyl-CoA + HCO(3)(-) = ADP + phosphate + malonyl-CoA.

-!- This enzyme is a multi-domain polypeptide that catalyzes three different activities - a biotin carboxyl-carrier protein (BCCP), a biotin carboxylase that catalyzes the transfer of a carboxyl group from hydrogencarbonate to the biotin molecule carried by the carrier protein, and the transfer of the carboxyl group from biotin to acetyl-CoA, forming malonyl-CoA. -!- In some organisms these activities are catalyzed by separate enzymes (see EC 6.3.4.14 and EC 2.1.3.15). -!- The carboxylation of the carrier protein requires ATP, while the transfer of the carboxyl group to acetyl-CoA does not.

UniProtKB Entries (1)

Q00955

ACAC_YEAST

Saccharomyces cerevisiae S288C

Acetyl-CoA carboxylase

PDB Structure

PDB	3H0J
External Links	PDBSum Proteopedia
Method	X-RAY DIFFRACTION
Organism
Primary Citation	Discovery of small molecule isozyme non-specific inhibitors of mammalian acetyl-CoA carboxylase 1 and 2. Corbett, J.W., Freeman-Cook, K.D., Elliott, R., Vajdos, F., Rajamohan, F., Kohls, D., Marr, E., Zhang, H., Tong, L., Tu, M., Murdande, S., Doran, S.D., Houser, J.A., Song, W., Jones, C.J., Coffey, S.B., Buzon, L., Minich, M.L., Dirico, K.J., Tapley, S., McPherson, R.K., Sugarman, E., Harwood, H.J., Esler, W. Bioorg.Med.Chem.Lett.