CATH Domain 3g1nA03

CATH Classification

Level	CATH Code	Description
	3	Alpha Beta
	3.30	2-Layer Sandwich
	3.30.2410	Hect, E3 ligase catalytic fold
	3.30.2410.10	Hect, E3 ligase catalytic domain

Domain Context

CATH Clusters

Superfamily	Hect, E3 ligase catalytic domain
Functional Family	E3 ubiquitin-protein ligase HUWE1, variant

Enzyme Information

2.3.2.26

HECT-type E3 ubiquitin transferase.

based on mapping to UniProt Q7Z6Z7

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)- ubiquitinyl-[acceptor protein]-L-lysine.

-!- In the first step the enzyme transfers ubiquitin from the E2 ubiquitin-conjugating enzyme (EC 2.3.2.23) to a cysteine residue in its HECT domain (which is located in the C-terminal region), forming a thioester bond. -!- In a subsequent step the enzyme transfers the ubiquitin to an acceptor protein, resulting in the formation of an isopeptide bond between the C-terminal glycine residue of ubiquitin and the epsilon- amino group of an L-lysine residue of the acceptor protein. -!- Cf. EC 2.3.2.27 and EC 2.3.2.31.

UniProtKB Entries (1)

Q7Z6Z7

HUWE1_HUMAN

Homo sapiens

E3 ubiquitin-protein ligase HUWE1

PDB Structure

PDB	3G1N
External Links	PDBSum Proteopedia
Method	X-RAY DIFFRACTION
Organism
Primary Citation	Hect Domain of Human HUWE1/MULE Walker, J.R., Qiu, L., Li, Y., Davis, T., Tempel, W., Weigelt, J., Bountra, C., Arrowsmith, C.H., Edwards, A.M., Botchkarev, A., Dhe-Paganon, S. To be Published