CATH Classification
Domain Context
CATH Clusters
| Superfamily | DNA Cyclobutane Dipyrimidine Photolyase, subunit A, domain 3 |
| Functional Family | Cryptochrome-1 |
Enzyme Information
| 4.1.99.13 |
(6-4)DNA photolyase.
based on mapping to UniProt O48652
(6-4) photoproduct (in DNA) = 2 pyrimidine residues (in DNA).
-!- The overall repair reaction consists of two distinct steps, one of which is light-independent and the other one light-dependent. -!- In the initial light-independent step, a 6-iminium ion is thought to be generated via proton transfer induced by two histidines highly conserved among the (6-4) photolyases. -!- This intermediate spontaneously rearranges to form an oxetane intermediate by intramolecular nucleophilic attack. -!- In the subsequent light-driven reaction, one electron is believed to be transferred from the fully reduced FAD cofactor (FADH(-)) to the oxetane intermediate thus forming a neutral FADH radical and an anionic oxetane radical, which spontaneously fractures. -!- The excess electron is then back-transferred to the flavin radical restoring the fully reduced flavin cofactor and a pair of pyrimidine bases.
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UniProtKB Entries (1)
| O48652 |
UVR3_ARATH
Arabidopsis thaliana
(6-4)DNA photolyase
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PDB Structure
| PDB | 3FY4 |
| External Links | |
| Method | X-RAY DIFFRACTION |
| Organism | |
| Primary Citation |
Functional motifs in the (6-4) photolyase crystal structure make a comparative framework for DNA repair photolyases and clock cryptochromes.
Proc.Natl.Acad.Sci.USA
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