CATH Classification
| Level | CATH Code | Description |
|---|---|---|
|
3 | Alpha Beta |
|
3.40 | 3-Layer(aba) Sandwich |
|
3.40.50 | Rossmann fold |
|
3.40.50.720 | NAD(P)-binding Rossmann-like Domain |
Domain Context
CATH Clusters
| Superfamily | NAD(P)-binding Rossmann-like Domain |
| Functional Family |
Enzyme Information
| 1.3.1.76 |
Precorrin-2 dehydrogenase.
based on mapping to UniProt P61818
Precorrin-2 + NAD(+) = sirohydrochlorin + NADH.
-!- Catalyzes the second of three steps leading to the formation of siroheme from uroporphyrinogen III. -!- The first step involves the donation of two S-adenosyl-L-methionine- derived methyl groups to carbons 2 and 7 of uroporphyrinogen III to form precorrin-2 (EC 2.1.1.107) and the third step involves the chelation of ferrous iron to sirohydrochlorin to form siroheme (EC 4.99.1.4). -!- In Saccharomyces cerevisiae, the last two steps are carried out by a single bifunctional enzyme, Met8p. -!- In some bacteria, steps 1-3 are catalyzed by a single multifunctional protein called CysG, whereas in Bacillus megaterium, three separate enzymes carry out each of the steps, with SirC being responsible for the above reaction.
|
UniProtKB Entries (1)
| P61818 |
SIRC_BACME
Bacillus megaterium
Precorrin-2 dehydrogenase
|
PDB Structure
| PDB | 3DFZ |
| External Links | |
| Method | X-RAY DIFFRACTION |
| Organism | |
| Primary Citation |
Structure and function of SirC from Bacillus megaterium: a metal-binding precorrin-2 dehydrogenase
Biochem.J.
|