CATH Classification

Domain Context

CATH Clusters

Superfamily Cholesterol Oxidase; domain 2
Functional Family

Enzyme Information

5.3.3.1
Steroid Delta-isomerase.
based on mapping to UniProt P12676
A 3-oxo-Delta(5)-steroid = a 3-oxo-Delta(4)-steroid.
1.1.3.6
Cholesterol oxidase.
based on mapping to UniProt P12676
Cholesterol + O(2) = cholest-5-en-3-one + H(2)O(2).
-!- Cholesterol oxidases are secreted bacterial bifunctional enzymes that catalyze the first two steps in the degradation of cholesterol. -!- The enzyme catalyzes the oxidation of the 3-beta-hydroxyl group to a keto group, and the isomerization of the double bond in the oxidized steroid ring system from the Delta(5) position to the Delta(6) position (cf. EC 5.3.3.1).

UniProtKB Entries (1)

P12676
CHOD_STRS0
Streptomyces sp. SA-COO
Cholesterol oxidase

PDB Structure

PDB 3B6D
External Links
Method X-RAY DIFFRACTION
Organism Escherichia
Primary Citation
Distortion of flavin geometry is linked to ligand binding in cholesterol oxidase
Lyubimov, A.Y., Heard, K., Tang, H., Sampson, N.S., Vrielink, A.
Protein Sci.
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