CATH Classification

Domain Context

CATH Clusters

Superfamily Glycosidases
Functional Family Beta-glucosidase 7

Enzyme Information

3.2.1.21
Beta-glucosidase.
based on mapping to UniProt Q9FYS3
Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.
-!- Wide specificity for beta-D-glucosides. -!- Some examples also hydrolyze one or more of the following: beta-D- galactosides, alpha-L-arabinosides, beta-D-xylosides and beta-D- fucosides.
3.2.1.182
4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl glucoside beta-D-glucosidase.
based on mapping to UniProt Q9FYS3
(1) (2R)-4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside + H(2)O = 2,4-dihydroxy-7-methoxy-2H-1,4- benzoxazin-3(4H)-one + D-glucose. (2) (2R)-4-hydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D- glucopyranoside + H(2)O = 2,4-dihydroxy-2H-1,4-benzoxazin-3(4H)-one + D-glucose.
-!- The enzyme from Triticum aestivum (wheat) has a higher affinity for DIMBOA glucoside than DIBOA glucoside. -!- With Secale cereale (rye) the preference is reversed.

UniProtKB Entries (1)

Q9FYS3
HGGL_SECCE
Secale cereale
4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl glucoside beta-D-glucosidase, chloroplastic

PDB Structure

PDB 3AIW
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Active-site architecture of benzoxazinone-glucoside beta-D-glucosidases in Triticeae
Sue, M., Nakamura, C., Miyamoto, T., Yajima, S.
Plant Sci.
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