CATH Domain 3afeA02

CATH Classification

Level	CATH Code	Description
	2	Mainly Beta
	2.40	Beta Barrel
	2.40.110	Butyryl-CoA Dehydrogenase, subunit A; domain 2
	2.40.110.10	Butyryl-CoA Dehydrogenase, subunit A, domain 2

Domain Context

CATH Clusters

Superfamily	Butyryl-CoA Dehydrogenase, subunit A, domain 2
Functional Family	Flavin-dependent monooxygenase, oxygenase subunit

Enzyme Information

1.14.14.12

3-hydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione monooxygenase.

based on mapping to UniProt P9WJA0

3-hydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione + FMNH(2) + O(2) = 3,4-dihydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione + FMN + H(2)O.

-!- This bacterial enzyme participates in the degradation of several steroids, including cholesterol and testosterone. -!- It can use either FADH or FMNH(2) as flavin cofactor. -!- The enzyme forms a two-component system with a reductase (HsaB) that utilizes NADH to reduce the flavin, which is then transferred to the oxygenase subunit.

UniProtKB Entries (2)

P9WJA1	HSAA_MYCTU Mycobacterium tuberculosis H37Rv Flavin-dependent monooxygenase, oxygenase subunit HsaA
P9WJA0	HSAA_MYCTO Mycobacterium tuberculosis CDC1551 Flavin-dependent monooxygenase, oxygenase subunit HsaA

PDB Structure

PDB	3AFE
External Links	PDBSum Proteopedia
Method	X-RAY DIFFRACTION
Organism
Primary Citation	A flavin-dependent monooxygenase from Mycobacterium tuberculosis involved in cholesterol catabolism Dresen, C., Lin, L.Y., D'Angelo, I., Tocheva, E.I., Strynadka, N., Eltis, L.D. J.Biol.Chem.