CATH Domain 3afeA01

CATH Classification

Level	CATH Code	Description
	1	Mainly Alpha
	1.10	Orthogonal Bundle
	1.10.540	Butyryl-Coa Dehydrogenase, subunit A; domain 1
	1.10.540.10	Acyl-CoA dehydrogenase/oxidase, N-terminal domain

Domain Context

CATH Clusters

Superfamily	Acyl-CoA dehydrogenase/oxidase, N-terminal domain
Functional Family	Flavin-dependent monooxygenase, oxygenase subunit HsaA

Enzyme Information

1.14.14.12

3-hydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione monooxygenase.

based on mapping to UniProt P9WJA0

3-hydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione + FMNH(2) + O(2) = 3,4-dihydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione + FMN + H(2)O.

-!- This bacterial enzyme participates in the degradation of several steroids, including cholesterol and testosterone. -!- It can use either FADH or FMNH(2) as flavin cofactor. -!- The enzyme forms a two-component system with a reductase (HsaB) that utilizes NADH to reduce the flavin, which is then transferred to the oxygenase subunit.

UniProtKB Entries (2)

P9WJA1	HSAA_MYCTU Mycobacterium tuberculosis H37Rv Flavin-dependent monooxygenase, oxygenase subunit HsaA
P9WJA0	HSAA_MYCTO Mycobacterium tuberculosis CDC1551 Flavin-dependent monooxygenase, oxygenase subunit HsaA

PDB Structure

PDB	3AFE
External Links	PDBSum Proteopedia
Method	X-RAY DIFFRACTION
Organism
Primary Citation	A flavin-dependent monooxygenase from Mycobacterium tuberculosis involved in cholesterol catabolism Dresen, C., Lin, L.Y., D'Angelo, I., Tocheva, E.I., Strynadka, N., Eltis, L.D. J.Biol.Chem.