CATH Classification

Domain Context

CATH Clusters

Superfamily endopeptidase domain like (from Nostoc punctiforme)
Functional Family Bifunctional glutathionylspermidine synthetase/amidase

Enzyme Information

3.5.1.78
Glutathionylspermidine amidase.
based on mapping to UniProt P0AES0
Glutathionylspermidine + H(2)O = glutathione + spermidine.
-!- Transforms glutathionylspermidine into glutathione and spermidine. -!- The enzyme from Escherichia coli is bifunctional and also catalyzes the reaction of EC 6.3.1.8, resulting in a net hydrolysis of ATP.
6.3.1.8
Glutathionylspermidine synthase.
based on mapping to UniProt P0AES0
Glutathione + spermidine + ATP = glutathionylspermidine + ADP + phosphate.
-!- Involved in the synthesis of trypanothione in trypanosomatids. -!- The enzyme from Escherichia coli is bifunctional and also catalyzes the EC 3.5.1.78 reaction, resulting in a net hydrolysis of ATP.

UniProtKB Entries (1)

P0AES0
GSP_ECOLI
Escherichia coli K-12
Bifunctional glutathionylspermidine synthetase/amidase

PDB Structure

PDB 3A30
External Links
Method X-RAY DIFFRACTION
Organism
Primary Citation
Protein S-thiolation by Glutathionylspermidine (Gsp): the role of Escherichia coli Gsp synthetASE/amidase in redox regulation
Chiang, B.-Y., Chen, T.-C., Pai, C.-H., Chou, C.-C., Chen, H.-H., Ko, T.-P., Hsu, W.-H., Chang, C.-Y., Wu, W.-F., Wang, A.H.-J., Lin, C.-H.
J.Biol.Chem.
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