CATH Classification
Domain Context
CATH Clusters
| Superfamily | Spore Coat Polysaccharide Biosynthesis Protein SpsA; Chain A |
| Functional Family |
Enzyme Information
| 2.4.1.226 |
N-acetylgalactosaminyl-proteoglycan 3-beta-glucuronosyltransferase.
based on mapping to UniProt Q8L0V4
(1) UDP-alpha-D-glucuronate + [protein]-3-O-(beta-D-GalNAc-(1->4)-beta-D- GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-serine = UDP + [protein]-3-O-(beta-D-GlcA-(1->3)-beta-D-GalNAc-(1->4)-beta-D-GlcA- (1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-serine. (2) UDP-alpha-D-glucuronate + [protein]-3-O-((beta-D-GalNAc-(1->4)-beta- D-GlcA-(1->3))(n)-beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-Gal- (1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-serine = UDP + [protein]-3- O-(beta-D-GlcA-(1->3)-(beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3))(n)-beta- D-GalNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)- beta-D-Xyl)-L-serine.
-!- Involved in the biosynthesis of chondroitin and dermatan sulfate. -!- The human chondroitin synthetase is a bifunctional glycosyltransferase, which also has the EC 2.4.1.175 activity required for the synthesis of the chondroitin sulfate disaccharide repeats. -!- Similar chondroitin synthase 'copolymerases' can be found in Pasteurella multocida and Escherichia coli. -!- There is also another human protein with apparently only the 3-beta- glucuronosyltransferase activity.
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| 2.4.1.175 |
Glucuronosyl-N-acetylgalactosaminyl-proteoglycan 4-beta-N- acetylgalactosaminyltransferase.
based on mapping to UniProt Q8L0V4
(1) UDP-N-acetyl-alpha-D-galactosamine + [protein]-3-O-(beta-D-GlcA- (1->3)-beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D- Gal-(1->4)-beta-D-Xyl)-L-serine = UDP + [protein]-3-O-(beta-D-GalNAc- (1->4)-beta-D-GlcA-(1->3)-beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D- Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-serine. (2) UDP-N-acetyl-alpha-D-galactosamine + [protein]-3-O-(beta-D-GlcA- (1->3)-(beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3))(n)-beta-D-GalNAc-(1->4)- beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L- serine = UDP + [protein]-3-O-((beta-D-GalNAc-(1->4)-beta-D-GlcA- (1->3))(n+1)-beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)- beta-D-Gal-(1->4)-beta-D-Xyl)-L-serine.
-!- Involved in the biosynthesis of chondroitin sulfate. -!- The human form of this enzyme is a bifunctional glycosyltransferase, which also has the EC 2.4.1.226 activity required for the synthesis of the chondroitin sulfate disaccharide repeats. -!- Similar chondroitin synthase 'copolymerases' can be found in Pasteurella multocida and Escherichia coli.
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UniProtKB Entries (1)
| Q8L0V4 |
CHS_ECOLX
Escherichia coli
Chondroitin synthase
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PDB Structure
| PDB | 2Z86 |
| External Links | |
| Method | X-RAY DIFFRACTION |
| Organism | |
| Primary Citation |
Crystal structure of chondroitin polymerase from Escherichia coli K4.
Biochem. Biophys. Res. Commun.
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