1 keywords

CATH Domain 2z5dA00

CATH Classification

Level CATH Code Description
Class 3 Alpha Beta
Architecture 3.10 Roll
Topology 3.10.110 Ubiquitin Conjugating Enzyme
Homologous Superfamily 3.10.110.10 Ubiquitin Conjugating Enzyme

Domain Context

CATH Clusters

Superfamily Ubiquitin Conjugating Enzyme
Functional Family ubiquitin-conjugating enzyme E2 H isoform X2

Enzyme Information

2.3.2.23
E2 ubiquitin-conjugating enzyme.
based on mapping to UniProt P62256
S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L- cysteine.
-!- The E2 ubiquitin-conjugating enzyme acquires the activated ubquitin from the E1 ubiquitin-activating enzyme (EC 6.2.1.45) and binds it via a transthioesterification reaction to itself. -!- In the human enzyme the catalytic center is located at Cys-87 where ubiquitin is bound via its C-terminal glycine in a thioester linkage. -!- Formerly EC 6.3.2.19.
2.3.2.24
(E3-independent) E2 ubiquitin-conjugating enzyme.
based on mapping to UniProt P62256
S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E1 ubiquitin-activating enzyme]-L-cysteine + N(6)- monoubiquitinyl-[acceptor protein]-L-lysine.
-!- The enzyme transfers a single ubiquitin directly from an ubiquitinated E1 ubiquitin-activating enzyme to itself, and on to a lysine residue of the acceptor protein without involvement of E3 ubiquitin transferases (cf. EC 2.3.2.26, EC 2.3.2.27). -!- It forms a labile ubiquitin adduct in the presence of E1, ubiquitin, and Mg(2+)-ATP and catalyzes the conjugation of ubiquitin to protein substrates, independently of E3. -!- This transfer has only been observed with small proteins. -!- In vitro a transfer to small acceptors (e.g. L-lysine, N-acetyl-L- lysine methyl ester) has been observed.

UniProtKB Entries (1)

P62256
UBE2H_HUMAN
Homo sapiens
Ubiquitin-conjugating enzyme E2 H

PDB Structure

PDB 2Z5D
External Links
Method X-RAY DIFFRACTION
Organism Escherichia
Primary Citation
A human ubiquitin conjugating enzyme (E2)-HECT E3 ligase structure-function screen.
Sheng, Y., Hong, J.H., Doherty, R., Srikumar, T., Shloush, J., Avvakumov, G.V., Walker, J.R., Xue, S., Neculai, D., Wan, J.W., Kim, S.K., Arrowsmith, C.H., Raught, B., Dhe-Paganon, S.
MOL.CELL PROTEOMICS