CATH Domain 2xbbA01

CATH Classification

Level	CATH Code	Description
	3	Alpha Beta
	3.90	Alpha-Beta Complex
	3.90.1750	Hect, E3 ligase catalytic domain fold
	3.90.1750.10	Hect, E3 ligase catalytic domains

Domain Context

CATH Clusters

Superfamily	Hect, E3 ligase catalytic domains
Functional Family	E3 ubiquitin-protein ligase NEDD4-like

Enzyme Information

2.3.2.26

HECT-type E3 ubiquitin transferase.

based on mapping to UniProt P46934

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)- ubiquitinyl-[acceptor protein]-L-lysine.

-!- In the first step the enzyme transfers ubiquitin from the E2 ubiquitin-conjugating enzyme (EC 2.3.2.23) to a cysteine residue in its HECT domain (which is located in the C-terminal region), forming a thioester bond. -!- In a subsequent step the enzyme transfers the ubiquitin to an acceptor protein, resulting in the formation of an isopeptide bond between the C-terminal glycine residue of ubiquitin and the epsilon- amino group of an L-lysine residue of the acceptor protein. -!- Cf. EC 2.3.2.27 and EC 2.3.2.31.

UniProtKB Entries (2)

P63048	RL40_BOVIN Bos taurus Ubiquitin-60S ribosomal protein L40
P46934	NEDD4_HUMAN Homo sapiens E3 ubiquitin-protein ligase NEDD4

PDB Structure

PDB	2XBB
External Links	PDBSum Proteopedia
Method	X-RAY DIFFRACTION
Organism
Primary Citation	Structure of the Hect:Ubiquitin Complex and its Role in Ubiquitin Chain Elongation Maspero, E., Mari, S., Valentini, E., Musacchio, A., Fish, A., Pasqualato, S., Polo, S. Embo Rep.