CATH Classification
| Level | CATH Code | Description |
|---|---|---|
|
1 | Mainly Alpha |
|
1.10 | Orthogonal Bundle |
|
1.10.1040 | N-(1-d-carboxylethyl)-l-norvaline Dehydrogenase; domain 2 |
|
1.10.1040.50 |
Domain Context
CATH Clusters
| Superfamily | 1.10.1040.50 |
| Functional Family | Peroxisomal bifunctional enzyme |
Enzyme Information
| 1.1.1.35 |
3-hydroxyacyl-CoA dehydrogenase.
based on mapping to UniProt P07896
(S)-3-hydroxyacyl-CoA + NAD(+) = 3-oxoacyl-CoA + NADH.
-!- Also oxidizes S-3-hydroxyacyl-N-acylthioethanolamine and S-3- hydroxyacylhydrolipoate. -!- Some enzymes act, more slowly, with NADP(+). -!- Broad specificity to acyl chain-length (cf. EC 1.1.1.211).
|
| 4.2.1.17 |
Enoyl-CoA hydratase.
based on mapping to UniProt P07896
(3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H(2)O.
-!- Acts in the reverse direction. -!- With cis-compounds, yields (3R)-3-hydroxyacyl-CoA (cf. EC 4.2.1.74).
|
| 5.3.3.8 |
Delta(3)-Delta(2)-enoyl-CoA isomerase.
based on mapping to UniProt P07896
(1) A (3Z)-alk-3-enoyl-CoA = a (2E)-alk-2-enoyl-CoA. (2) A (3E)-alk-3-enoyl-CoA = a (2E)-alk-2-enoyl-CoA.
-!- The enzyme participates in the beta-oxidation of fatty acids with double bonds at an odd position. -!- Processing of these substrates via the beta-oxidation system results in intermediates with a cis- or trans-double bond at position C(3), which cannot be processed further by the regular enzymes of the beta- oxidation system. -!- This enzyme isomerizes the bond to a trans bond at position C(2), which can be processed further. -!- The reaction rate is ten times higher for the (3Z) isomers than for (3E) isomers. -!- The enzyme can also catalyze the isomerization of 3-acetylenic fatty acyl thioesters to 2,3-dienoyl fatty acyl thioesters.
|
UniProtKB Entries (1)
| P07896 |
ECHP_RAT
Rattus norvegicus
Peroxisomal bifunctional enzyme
|
PDB Structure
| PDB | 2X58 |
| External Links | |
| Method | X-RAY DIFFRACTION |
| Organism | |
| Primary Citation |
The Crystal Structure of Liganded Rat Peroxisomal Multifunctional Enzyme Type 1: A Flexible Molecule with Two Interconnected Active Sites
J.Biol.Chem.
|
