CATH Domain 2wwuA02

CATH Classification

Level	CATH Code	Description
	1	Mainly Alpha
	1.20	Up-down Bundle
	1.20.58	Methane Monooxygenase Hydroxylase; Chain G, domain 1
	1.20.58.1360

Domain Context

CATH Clusters

Superfamily	1.20.58.1360
Functional Family	Histone lysine demethylase PHF8

Enzyme Information

1.14.11.27

[Histone H3]-dimetyl-L-lysine-36 demethylase.

based on mapping to UniProt Q9UPP1

[Protein]-N(6),N(6)-dimethyl-L-lysine + 2 2-oxoglutarate + 2 O(2) = [protein]-L-lysine + 2 succinate + 2 formaldehyde + 2 CO(2).

-!- Of the seven potential methylation sites in histones H3 (K4, K9, K27, K36, K79) and H4 (K20, R3) from HeLa cells, the enzyme is specific for Lys-36. -!- Lysine residues exist in three methylation states (mono-, di- and trimethylated). -!- The enzyme preferentially demethylates the dimethyl form of Lys-36 (K36me2), which is its natural substrate, to form the monomethyl and unmethylated forms of Lys-36. -!- It can also demethylate the monomethyl- but not the trimethyl form of Lys-36.

UniProtKB Entries (1)

Q9UPP1

PHF8_HUMAN

Homo sapiens

Histone lysine demethylase PHF8

PDB Structure

PDB	2WWU
External Links	PDBSum Proteopedia
Method	X-RAY DIFFRACTION
Organism
Primary Citation	Crystal structure of the PHF8 Jumonji domain, an Nepsilon-methyl lysine demethylase. Yue, W.W., Hozjan, V., Ge, W., Loenarz, C., Cooper, C.D., Schofield, C.J., Kavanagh, K.L., Oppermann, U., McDonough, M.A. FEBS Lett.