CATH Classification
| Level | CATH Code | Description |
|---|---|---|
|
1 | Mainly Alpha |
|
1.10 | Orthogonal Bundle |
|
1.10.630 | Cytochrome p450 |
|
1.10.630.10 | Cytochrome P450 |
Domain Context
CATH Clusters
| Superfamily | Cytochrome P450 |
| Functional Family | Cytochrome P450 monooxygenase PikC |
Enzyme Information
| 1.14.15.33 |
Pikromycin synthase.
based on mapping to UniProt O87605
(1) Narbomycin + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H(+) + O(2) = pikromycin + 2 oxidized ferredoxin [iron-sulfur] cluster + H(2)O. (2) Narbomycin + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H(+) + O(2) = neopikromycin + 2 oxidized ferredoxin [iron-sulfur] cluster + H(2)O. (3) Narbomycin + 4 reduced ferredoxin [iron-sulfur] cluster + 4 H(+) + 2 O(2) = novapikromyin + 4 oxidized ferredoxin [iron-sulfur] cluster + 2 H(2)O. (4) 10-deoxymethymycin + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H(+) + O(2) = methymycin + 2 oxidized ferredoxin [iron-sulfur] cluster + H(2)O. (5) 10-deoxymethymycin + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H(+) + O(2) = neomethymycin + 2 oxidized ferredoxin [iron-sulfur] cluster + H(2)O. (6) 10-deoxymethymycin + 4 reduced ferredoxin [iron-sulfur] cluster + 4 H(+) + 2 O(2) = novamethymycin + 4 oxidized ferredoxin [iron-sulfur] cluster + 2 H(2)O.
-!- A cytochrome P450 (heme-thiolate) protein. -!- Involved in the biosynthesis of a number of bacterial macrolide antibiotics containing a desosamine glycoside unit. -!- With narbomycin it hydroxylates at either C-12 to give pikromycin or C-14 to give neopikromycin or both positions to give narvopikromycin. -!- With 10-deoxymethymycin it hydroxylates at either C-10 to give methymycin or C-12 to give neomethymycin or both positions to give novamethymycin. -!- Formerly EC 1.14.13.185.
|
UniProtKB Entries (1)
| O87605 |
PIKC_STRVZ
Streptomyces venezuelae
Cytochrome P450 monooxygenase PikC
|
PDB Structure
| PDB | 2WI9 |
| External Links | |
| Method | X-RAY DIFFRACTION |
| Organism | |
| Primary Citation |
Selective Oxidation of Carbolide C-H Bonds by an Engineered Macrolide P450 Mono-Oxygenase.
Proc.Natl.Acad.Sci.USA
|
