CATH Domain 2qyuA02

CATH Classification

Level	CATH Code	Description
	1	Mainly Alpha
	1.25	Alpha Horseshoe
	1.25.40	Serine Threonine Protein Phosphatase 5, Tetratricopeptide repeat
	1.25.40.300	Putative secreted effector protein

Domain Context

CATH Clusters

Superfamily	Putative secreted effector protein
Functional Family	SPI-1 type III secretion system effector HECT-type E3 ubiquitin transferase SopA

Enzyme Information

2.3.2.26

HECT-type E3 ubiquitin transferase.

based on mapping to UniProt Q8ZNR3

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)- ubiquitinyl-[acceptor protein]-L-lysine.

-!- In the first step the enzyme transfers ubiquitin from the E2 ubiquitin-conjugating enzyme (EC 2.3.2.23) to a cysteine residue in its HECT domain (which is located in the C-terminal region), forming a thioester bond. -!- In a subsequent step the enzyme transfers the ubiquitin to an acceptor protein, resulting in the formation of an isopeptide bond between the C-terminal glycine residue of ubiquitin and the epsilon- amino group of an L-lysine residue of the acceptor protein. -!- Cf. EC 2.3.2.27 and EC 2.3.2.31.

UniProtKB Entries (1)

Q8ZNR3

SOPA_SALTY

Salmonella enterica subsp. enterica serovar Typhimurium str. LT2

E3 ubiquitin-protein ligase SopA

PDB Structure

PDB	2QYU
External Links	PDBSum Proteopedia
Method	X-RAY DIFFRACTION
Organism	Escherichia
Primary Citation	Crystal structure of SopA, a Salmonella effector protein mimicking a eukaryotic ubiquitin ligase. Diao, J., Zhang, Y., Huibregtse, J.M., Zhou, D., Chen, J. Nat.Struct.Mol.Biol.