CATH Classification

Domain Context

CATH Clusters

Superfamily FAD/NAD(P)-binding domain
Functional Family

Enzyme Information

1.14.19.9
Tryptophan 7-halogenase.
based on mapping to UniProt Q8KHZ8
Tryptophan + FADH(2) + chloride + O(2) + H(+) = 7-chloro-L-tryptophan + FAD + 2 H(2)O.
-!- A flavin-dependent halogenase. -!- The enzyme from the bacterium Lechevalieria aerocolonigenes catalyzes the initial step in the biosynthesis of rebeccamycin. -!- It utilizes molecular oxygen to oxidize the FADH(2) cofactor, giving C4a-hydroperoxyflavin, which then reacts with chloride to produce a hypochlorite ion. -!- The latter reacts with an active site lysine to generate a chloramine, which chlorinates the substrate. -!- Also acts on bromide ion. -!- Cf. EC 1.14.19.58 and EC 1.14.19.59.

UniProtKB Entries (1)

Q8KHZ8
REBH_NOCAE
Lechevalieria aerocolonigenes
Flavin-dependent tryptophan halogenase RebH

PDB Structure

PDB 2OA1
External Links
Method X-RAY DIFFRACTION
Organism Escherichia
Primary Citation
The structure of flavin-dependent tryptophan 7-halogenase RebH.
Bitto, E., Huang, Y., Bingman, C.A., Singh, S., Thorson, J.S., Phillips Jr., G.N.
Proteins