CATH Classification
| Level | CATH Code | Description |
|---|---|---|
|
1 | Mainly Alpha |
|
1.10 | Orthogonal Bundle |
|
1.10.1200 | Non-ribosomal Peptide Synthetase Peptidyl Carrier Protein; Chain A |
|
1.10.1200.10 | ACP-like |
Domain Context
CATH Clusters
| Superfamily | ACP-like |
| Functional Family | Probable polyketide synthase pks17 |
Enzyme Information
| 2.3.1.94 |
6-deoxyerythronolide-B synthase.
based on mapping to UniProt Q03131
Propanoyl-CoA + 6 (2S)-methylmalonyl-CoA + 6 NADPH = 6-deoxyerythronolide B + 7 CoA + 6 CO(2) + H(2)O + 6 NADP(+).
-!- The product, 6-deoxyerythronolide B, contains a 14-membered lactone ring and is an intermediate in the biosynthesis of erythromycin antibiotics. -!- Biosynthesis of 6-deoxyerythronolide B requires 28 active sites that are precisely arranged along three large polypeptides, denoted DEBS1, -2 and -3. -!- The polyketide product is synthesized by the processive action of a loading didomain, six extension modules and a terminal thioesterase domain. -!- Each extension module contains a minimum of a ketosynthase (KS), an acyltransferase (AT) and an acyl-carrier protein (ACP). -!- The KS domain both accepts the growing polyketide chain from the previous module and catalyzes the subsequent decarboxylative condensation between this substrate and an ACP-bound methylmalonyl extender unit, introduce by the AT domain. -!- This combined effort gives rise to a new polyketide intermediate that has been extended by two carbon atoms.
|
UniProtKB Entries (1)
| Q03131 |
ERYA1_SACER
Saccharopolyspora erythraea
6-deoxyerythronolide-B synthase EryA1, modules 1 and 2
|
PDB Structure
| PDB | 2JU1 |
| External Links | |
| Method | SOLUTION NMR |
| Organism | Escherichia |
| Primary Citation |
Solution structure and proposed domain domain recognition interface of an acyl carrier protein domain from a modular polyketide synthase.
Protein Sci.
|
