CATH Classification
| Level | CATH Code | Description |
|---|---|---|
|
3 | Alpha Beta |
|
3.40 | 3-Layer(aba) Sandwich |
|
3.40.109 | NADH Oxidase |
|
3.40.109.10 | NADH Oxidase |
Domain Context
CATH Clusters
| Superfamily | NADH Oxidase |
| Functional Family | 5,6-dimethylbenzimidazole synthase |
Enzyme Information
| 1.13.11.79 |
5,6-dimethylbenzimidazole synthase.
based on mapping to UniProt Q92PC8
FMNH(2) + NADH + O(2) = 5,6-dimethylbenzimidazole + D-erythrose 4-phosphate + NAD(+) + other product(s).
-!- The enzyme catalyzes a complex oxygen-dependent conversion of reduced flavin mononucleotide to form 5,6-dimethylbenzimidazole, the lower ligand of vitamin B12. -!- This conversion involves many sequential steps in two distinct stages, and an alloxan intermediate that acts as a proton donor, a proton acceptor, and a hydride acceptor. -!- The C-2 of 5,6-dimethylbenzimidazole is derived from C-1' of the ribityl group of FMNH(2) and 2-H from the ribityl 1'-pro-S hydrogen. -!- While D-erythrose 4-phosphate has been shown to be one of the byproducts, the nature of the other product(s) has not been verified yet. -!- Formerly EC 1.14.99.40.
|
UniProtKB Entries (1)
| Q92PC8 |
BLUB_RHIME
Sinorhizobium meliloti 1021
5,6-dimethylbenzimidazole synthase
|
PDB Structure
| PDB | 2ISJ |
| External Links | |
| Method | X-RAY DIFFRACTION |
| Organism | Escherichia |
| Primary Citation |
BluB cannibalizes flavin to form the lower ligand of vitamin B12.
Nature
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